Bromodomains

Bromodomains (BRDs) are protein domains which recognize and bind to acetylated lysines.  Along with HATs and HDACsBRD proteins are emerging as a new class of druggable targets in cancer and inflammatory & cardiovascular diseases.

There are over 50 proteins containing one or more BRDs. BRDs have been identified in HATs, Methyltransferases (e.g. MLL), chromatin-remodeling complexes and transcriptional coactivators.

BRD inhibitor

(+)-JQ1 is a specific inhibitor of the BET family of acetyl-lysine recognition motifs, including the BRD2, BRD3, BRD4 and BRDT bromodomains. This inhibitor has been shown to inhibit BRD4 (BD1) with a Kd of 50 nM, and BRD4 (BD2) with a Kd of 90 nM.

Discover the (+)-JQ1 (Cat nr 27400) here

BRD active proteins

Directly access our ever-expanding list of BRD proteins here

Figure (right): Human Bromodomain containing 4  (Cat nr 31043), also known as BRD4, HUNK1, or MCAP, amino-acids 342-460 (GenBank Accession: NM_058243) with N-terminal His tag, MW = 14.7 kDa, expressed in an E. coli expression system.

BRD screening/profiling kits

Applications:

Inhibitor Screening Assay kit

ATAD2A Inhibitor Screening Assay Kit

ATAD2B Inhibitor Screening Assay Kit

BAZ2B Inhibitor Screening Assay Kit

BRD2 (BD2) Inhibitor Screening Assay Kit

BRD3 (BD1) Inhibitor Screening Assay Kit

BRD3 (BD1) Inhibitor Screening Assay Kit

BRD3 (BD2) Inhibitor Screening Assay Kit

BRD4 (BD1) Inhibitor Screening Assay Kit

TAF1 (BD1 + BD2) Inhibitor Screening Assay Kit

TAF1L (BD1 + BD2) Inhibitor Screening Assay Kit

TAF1L (BD2) Inhibitor Screening Assay Kit

 

Outsource your Bromodomain Screening and Profiling to evaluate the IC50 value of your compounds of interest.
Currently, our Bromodomain panel includes 11 bromodomain- containing proteins, including ATAD2A, BAZ2B, BRD2 (BD1), BRD2 (BD2), BRD2 (BD1 +BD2), BRD3 (BD1), BRD3 (BD2), BRD4 (BD1), BRD4 (BD2), BRD4 (BD1 + BD2) and BRDT.

For more information regarding our Bromodomain profiling services, get in contact with us!

Bromodomain ligands

  Peptide Acetylated et Lysine: Suitable for use as: Cat nr
BET Bromodomain Ligand Histone H4 (1-21) Lys 5, 8, 12 & 16 Ligand for several bromodomains including members of the BET bromodomain family: BRD2, BRD3, BRD4, and BRDT 33000
Bromodomain Ligand 2 Histone H3 (1-21) Lys 14 Ligand for several bromodomains including ATAD2 and BAZ2B 33003
Bromodomain Non-acetylated Ligand 1 Histone H4 (1-21) - Negative control for several bromodomains including members of the BET bromodomain family: BRD2, BRD3, BRD4, and BRDT 33005
Bromodomain Non-acetylated Ligand 2 Histone H3 (1-21) - Negative control for several bromodomains including BAZ2B

33004

 

Looking for specific research antibodies?

With our 180 000+ research antibodies rigourously selected from high quality manufacturers, you'll find all the primary antibodies you need for the analysis of your BRDs proteins of interest, or their related partners.
 
A few examples: Anti BRD2 Human Maxpab antibodyAnti BAZ2B antibody ... 
 
 Find your research antibodies easily with our dedicated antibody search engine!