Histone Methylation

Post-translational histone modifications impact the epigenetic regulation of transcription. Whereas histone acetylation has been well-characterized, the study of histone methylation is now becoming a hot research field for the scientific community.

Histone methyltransferases (HMTs) and their reverse partners histone demethylases are critical for transcriptional regulation in both normal and diseased states, and are important targets for drug discovery. Below is a table illustrating few examples of pairs of these mechanisms.

Histone Modifications Histone MethylTransferase (HMT) Histone demethylase
H3K4 MLLSET7/9, SMYD3 LSD1, JARID1A-D
H3K36 NSD1, SET2, SMYD2, 3 MJD2A-E, JHDM1A,
H3K79 Dot1L ?
H3K9 SUV39H1,2, G9a LSD1, JMJD2A-E
H4K20 SET8, SUV20H1,2 PHF8
H3K27 EZH1, EZH2 UTX, JMJD3
H3R PRMT1, PRMT3, PRMT5, PRMT6, PRMT7, CARM/PRM PADI1-4, JMJD6

 

 

 

 

 

 

Ever growing list of Histone methyltransferases (HMTs)

 

Some examples are displayed in the table to the right, click on the image to access the full list.

 

 

 

 

 


Activity measured with the SUV39H2 Direct Activity Assay Kit (Cat nr 52008) and purity of SUV39H2 (Cat nr 51080) lysine methyltransferase.

Ready-to-use functional methylation assays

Discover our unique portfolio of epigenetic assays kits for methylation research. These simple, sensitive assays provide precise measurement of methylation activity for drug discovery research.

Histone methylation of arginine (PRMT)

Chemiluminescent Assay Kit (96 Rxn) AlphaLISA Homogenous Assays (384 Rxn)
PRMT1 Assay kit PRMT1 Assay kit
PRMT3 Assay kit PRMT3 Assay kit
PRMT4 Assay kit PRMT5 Assay kit
PRMT5 Assay kit PRMT6 Assay kit
PRMT6 Assay kit PRMT8 Assay kit
   

 

 

 

 

 

Histone methylation of lysine (EZH2, G9a, Set7/9, SUV39H1/2....)

Chemiluminescent Assay Kit (96 Rxn) AlphaLISA Homogenous Assays (384 Rxn)
EZH2 Assay kit EZH2 Assay kit
G9a Assay kit G9a Assay kit
MLL1 complex Assay kit  
SETD2 Assay kit  
SUV39H1 Assay kit  
SUV39H2 Assay kit  

Histone Methyltransferase Screening and Profiling

Histone methyltransferase assays allow the determination of IC50 values of compounds. Our Histone methyltransferase panel now includes 16 histone methyltransferases:

EZH1, EZH2, G9a, GLP, MLL Complex, PRMT1, PRMT3, PRMT4, PRMT5, PRMT6, PRMT8, SETD2,SET7/9, SET8, SUV39H1 and SUV39H2.

If you are interested in sending us your compounds of interest to screen them on HMTs panel, just get in contact with us.

Get your specific research antibodies & ELISAs here...

With our 180 000+ research antibodies & ELISAs rigourously selected from high quality manufacturers, you'll find all the primary antibodies you need for the analysis of your Histone Methylation pathway.
 
A few examples: Anti Histone H3 (mono methyl Lys4)anti LSD1 antibody, anti PRMT3 antibodyGlobal Mono-Methyl Histone H4K20 Quantification Kit...
 
 Find your research antibodies easily with our dedicated antibody search engine!