PARP SilenciX®

Silencing: the most-adopted model to study gene function

The most classical approaches are based on siRNA expressed in the cell through different channels, like transient transfection of RNA Duplexes, of plasmids, or of viruses expressing RNA constructs. Whilst efficient, these strategies do suffer from the lack of stability. To overcome this issue, short hairpin (shRNA) can be used to increase stability, but they disrupt genome integrity.

Our solution to this technical challenge is SilenciX®, which combines RNAi technology, a DSIR program and a pEBV derived vector to generate ready-to-use knock-down clones in various fields of research, including epigenetics.

As demonstrated in numerous scientific publications, SilenciX® cell lines are used as a loss-of-function model.

SilenciX® is the promise of...

Ready-to-dispatch Poly ADP-Ribose polymerase SilenciX® cell lines

Designation % of KD* Cat nr
PARP1 97% 01-00019
PARP2 86% 01-00020
PARP3 Coming soon  
PARP9 Coming soon  
PARP14 Coming soon  
PARG 91% 01-0008

* tebu-bio quality controls are performed by qPCR

Contents of each SilenciX® kit

Example of functional assay performed with PARP1 SilenciX cell lines

PARP1 is known to be involved in DNA Damage Response, chromatin structure, transcription, RNA metabolism, cell death and cell cycle regulation.

Published data demonstrates that the knock-down or the inhibition of PARP1 results in a hypersensitivity of the cell to DNA damaging agents including Ionizing Radiation.
 


PARP1 SilenciX cell line noted PARP-1KD cells were found to be approximately 1.6 times more sensitive to the killing effects of IR than Control cells, based on the surviving fraction at 2 Gy